anti mcl 1 (Santa Cruz Biotechnology)
Structured Review
Anti Mcl 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcl+1/Mcl-1+Antibody/pm41899524-104-25-28
Average 96 stars, based on 2246 article reviews
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Nucleic Acid Electrophoresis:Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models. Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon®-P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from https://doi.org/10.3390/cells15020164 Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon ® -P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Membrane:Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models. Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon®-P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from https://doi.org/10.3390/cells15020164 Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon ® -P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Western Blot:Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models. Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon®-P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from https://doi.org/10.3390/cells15020164 Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Article Title: The Synergistic Effects of rhArg with Bcl-2 Inhibitors or Metformin Co-Treatment in Multiple Cancer Cell Models Article Snippet: After drug treatment, cells were harvested in RIPA buffer (Cat. R0278, Sigma Aldrich, St. Louis, MO, USA) supplemented with protease (Cat. 78430, Thermo Fisher, Rockford, IL, USA) and phosphatase (Cat. 78428, Thermo Fisher, Rockford, IL, USA) inhibitors. .. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to Immobilon ® -P PVDF Membrane (PVDF) (Cat. IPVH00010, Merck Millipore, Darmstadt, Germany) for Western blot analysis using the following primary antibodies: BAX (cst-2772), GAPDH (cst-2118), cleaved caspase 3 (cst-9664) and PARP (cst-9542) from Cell Signaling Technology (Danvers, MA, USA) or cyclin A (sc-239), CDK2 (sc-6248) and Article Title: Visomitin as a differentiation-inducing therapeutic agent through SYK inhibition in AML Article Snippet: N-acetylcysteine amide (NAC) was purchased from Sigma (Sigma, St. Louis, MO, United States; A9165). .. The following primary antibodies were used for western blots: Cyclin D1 (Santa Cruz Biotechnology, Dallas, TX, United States; sc-8396), CDK4 (Santa Cruz Biotechnology; sc-23896), p16 (Cell Signaling Technology, Danvers, MA, United States; 80772S), p21 (Cell Signaling Technology; 2947S), Rb (Santa Cruz Biotechnology; sc-102), Incubation:Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury. Article Snippet: .. After that the slides were incubated with primary antibodies against TOM20 (11802-1-AP, Proteintech), Fluorescence:Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury. Article Snippet: .. After that the slides were incubated with primary antibodies against TOM20 (11802-1-AP, Proteintech), In Vitro:Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury. Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ ml penicillin, 100 μg/ml streptomycin under 5% CO2 at 37°C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. BeforeH/R, the siRNAs specific to Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin under 5% CO 2 at 37 °C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. Before H/R, the siRNAs specific to Transfection:Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury. Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ ml penicillin, 100 μg/ml streptomycin under 5% CO2 at 37°C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. BeforeH/R, the siRNAs specific to Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin under 5% CO 2 at 37 °C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. Before H/R, the siRNAs specific to Knockdown:Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury. Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ ml penicillin, 100 μg/ml streptomycin under 5% CO2 at 37°C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. BeforeH/R, the siRNAs specific to Article Title: Targeting MCL1 by UMI-77 to induce mitophagy alleviates renal tubular epithelial cells injury in acute kidney injury Article Snippet: HK-2 cells were cultured in RPMI 1640 media (Sigma) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin under 5% CO 2 at 37 °C. .. HK-2 cells were subjected to 24 h of hypoxia followed by 3 h of reoxygenation to establish an in vitro hypoxia/reoxygenation (H/R) model. Before H/R, the siRNAs specific to |
